Review



goat polyclonal anti human tff2 antibody  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    R&D Systems goat polyclonal anti human tff2 antibody
    Fig. 2 Heterogeneity of the trefoil factor family 2 molecule in gastric juice, and its influence on the present assay method. a Recombinant trefoil factor family 2 <t>(TFF2)</t> (lane Ag) and patients’ gastric juices (lanes 1–4), treated with Laemmli buffer (without 2-mercap- toethanol), were loaded onto 4–20% gradient polyacrylamide gels. After electrophoresis, separated proteins were transferred electropho- retically onto a nitrocellulose membrane. The bands containing TFF2 were visualized by immunoblot analysis using anti-TFF2 antibody. b To investigate the effect of heterogeneity of the TFF2 molecule, we measured TFF2 levels in the serial dilutions of mixed-gastric juice samples that were prepared using two gastric juices (lanes 2 and 4 in a). Data are expressed as mean ± standard deviation (SD) derived from triplicate determinations in each of the two separate experiments
    Goat Polyclonal Anti Human Tff2 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+anti+human+tff2+antibody/TFF2+Antibody/pm21559742-57-10-15
    Average 93 stars, based on 5 article reviews
    goat polyclonal anti human tff2 antibody - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Pathophysiological investigation of the gastric surface mucous gel layer of patients with Helicobacter pylori infection by using immunoassays for trefoil factor family 2 and gastric gland mucous cell-type mucin in gastric juice."

    Article Title: Pathophysiological investigation of the gastric surface mucous gel layer of patients with Helicobacter pylori infection by using immunoassays for trefoil factor family 2 and gastric gland mucous cell-type mucin in gastric juice.

    Journal: Digestive diseases and sciences

    doi: 10.1007/s10620-011-1724-9

    Fig. 2 Heterogeneity of the trefoil factor family 2 molecule in gastric juice, and its influence on the present assay method. a Recombinant trefoil factor family 2 (TFF2) (lane Ag) and patients’ gastric juices (lanes 1–4), treated with Laemmli buffer (without 2-mercap- toethanol), were loaded onto 4–20% gradient polyacrylamide gels. After electrophoresis, separated proteins were transferred electropho- retically onto a nitrocellulose membrane. The bands containing TFF2 were visualized by immunoblot analysis using anti-TFF2 antibody. b To investigate the effect of heterogeneity of the TFF2 molecule, we measured TFF2 levels in the serial dilutions of mixed-gastric juice samples that were prepared using two gastric juices (lanes 2 and 4 in a). Data are expressed as mean ± standard deviation (SD) derived from triplicate determinations in each of the two separate experiments
    Figure Legend Snippet: Fig. 2 Heterogeneity of the trefoil factor family 2 molecule in gastric juice, and its influence on the present assay method. a Recombinant trefoil factor family 2 (TFF2) (lane Ag) and patients’ gastric juices (lanes 1–4), treated with Laemmli buffer (without 2-mercap- toethanol), were loaded onto 4–20% gradient polyacrylamide gels. After electrophoresis, separated proteins were transferred electropho- retically onto a nitrocellulose membrane. The bands containing TFF2 were visualized by immunoblot analysis using anti-TFF2 antibody. b To investigate the effect of heterogeneity of the TFF2 molecule, we measured TFF2 levels in the serial dilutions of mixed-gastric juice samples that were prepared using two gastric juices (lanes 2 and 4 in a). Data are expressed as mean ± standard deviation (SD) derived from triplicate determinations in each of the two separate experiments

    Techniques Used: Recombinant, Electrophoresis, Membrane, Western Blot, Standard Deviation, Derivative Assay

    Fig. 3 TFF2 concentration in gastric juices from healthy individuals and patients. a TFF2 concentration in gastric juices from patients with gastritis, gastric ulcer (GU), duodenal ulcer (DU) and healthy individuals (control). b TFF2 concentration in gastric juices from patients with H. pylori infection (HP (?)) and in H. pylori-negative patients (HP (-)). c TFF2 concentration in gastric juices from patients before and after the H. pylori eradication. *p \ 0.05; **p \ 0.01; NS not significant
    Figure Legend Snippet: Fig. 3 TFF2 concentration in gastric juices from healthy individuals and patients. a TFF2 concentration in gastric juices from patients with gastritis, gastric ulcer (GU), duodenal ulcer (DU) and healthy individuals (control). b TFF2 concentration in gastric juices from patients with H. pylori infection (HP (?)) and in H. pylori-negative patients (HP (-)). c TFF2 concentration in gastric juices from patients before and after the H. pylori eradication. *p \ 0.05; **p \ 0.01; NS not significant

    Techniques Used: Concentration Assay, Control, Infection

    Related Articles

    Incubation:

    Article Title: Pathophysiological investigation of the gastric surface mucous gel layer of patients with Helicobacter pylori infection by using immunoassays for trefoil factor family 2 and gastric gland mucous cell-type mucin in gastric juice.
    Article Snippet: .. Briefly, commercially available polystyrene immunoplates (Nunc, Roskilde, Denmark) were coated with goat polyclonal anti-human TFF2 antibody (2,000-fold dilution) (R&D systems, Minneapolis, MN) in 0.1 mol/l sodium bicarbonate, pH9.6 (1.0 mg protein/l) for 24 h at 4 C. Plates were washed four times with PBS-Tween after each of the subsequent incubation steps. .. Unoccupied sites were blocked with the blocking buffer (NOF Corp., Tokyo, Japan) for 24 h at 4 C. The prepared calibrators and samples were then added at 100 ll/well and incubated for 1 h at room temperature.



    Similar Products

    goat polyclonal anti human tff2 antibody  (R&D Systems)
    93
    R&D Systems goat polyclonal anti human tff2 antibody
    Fig. 2 Heterogeneity of the trefoil factor family 2 molecule in gastric juice, and its influence on the present assay method. a Recombinant trefoil factor family 2 <t>(TFF2)</t> (lane Ag) and patients’ gastric juices (lanes 1–4), treated with Laemmli buffer (without 2-mercap- toethanol), were loaded onto 4–20% gradient polyacrylamide gels. After electrophoresis, separated proteins were transferred electropho- retically onto a nitrocellulose membrane. The bands containing TFF2 were visualized by immunoblot analysis using anti-TFF2 antibody. b To investigate the effect of heterogeneity of the TFF2 molecule, we measured TFF2 levels in the serial dilutions of mixed-gastric juice samples that were prepared using two gastric juices (lanes 2 and 4 in a). Data are expressed as mean ± standard deviation (SD) derived from triplicate determinations in each of the two separate experiments
    Goat Polyclonal Anti Human Tff2 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+anti+human+tff2+antibody/TFF2+Antibody/pm21559742-57-10-15
    Average 93 stars, based on 1 article reviews
    goat polyclonal anti human tff2 antibody - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 2 Heterogeneity of the trefoil factor family 2 molecule in gastric juice, and its influence on the present assay method. a Recombinant trefoil factor family 2 (TFF2) (lane Ag) and patients’ gastric juices (lanes 1–4), treated with Laemmli buffer (without 2-mercap- toethanol), were loaded onto 4–20% gradient polyacrylamide gels. After electrophoresis, separated proteins were transferred electropho- retically onto a nitrocellulose membrane. The bands containing TFF2 were visualized by immunoblot analysis using anti-TFF2 antibody. b To investigate the effect of heterogeneity of the TFF2 molecule, we measured TFF2 levels in the serial dilutions of mixed-gastric juice samples that were prepared using two gastric juices (lanes 2 and 4 in a). Data are expressed as mean ± standard deviation (SD) derived from triplicate determinations in each of the two separate experiments

    Journal: Digestive diseases and sciences

    Article Title: Pathophysiological investigation of the gastric surface mucous gel layer of patients with Helicobacter pylori infection by using immunoassays for trefoil factor family 2 and gastric gland mucous cell-type mucin in gastric juice.

    doi: 10.1007/s10620-011-1724-9

    Figure Lengend Snippet: Fig. 2 Heterogeneity of the trefoil factor family 2 molecule in gastric juice, and its influence on the present assay method. a Recombinant trefoil factor family 2 (TFF2) (lane Ag) and patients’ gastric juices (lanes 1–4), treated with Laemmli buffer (without 2-mercap- toethanol), were loaded onto 4–20% gradient polyacrylamide gels. After electrophoresis, separated proteins were transferred electropho- retically onto a nitrocellulose membrane. The bands containing TFF2 were visualized by immunoblot analysis using anti-TFF2 antibody. b To investigate the effect of heterogeneity of the TFF2 molecule, we measured TFF2 levels in the serial dilutions of mixed-gastric juice samples that were prepared using two gastric juices (lanes 2 and 4 in a). Data are expressed as mean ± standard deviation (SD) derived from triplicate determinations in each of the two separate experiments

    Article Snippet: The biotinylated anti-human TFF2 antibody was prepared by using the goat polyclonal anti-human TFF2 antibody (R&D systems) and the EZ-Link Sulfo-NHS-LC-Biotinylation kit (Pierce, Rockford, IL) according to the manufacturer’s instructions.

    Techniques: Recombinant, Electrophoresis, Membrane, Western Blot, Standard Deviation, Derivative Assay

    Fig. 3 TFF2 concentration in gastric juices from healthy individuals and patients. a TFF2 concentration in gastric juices from patients with gastritis, gastric ulcer (GU), duodenal ulcer (DU) and healthy individuals (control). b TFF2 concentration in gastric juices from patients with H. pylori infection (HP (?)) and in H. pylori-negative patients (HP (-)). c TFF2 concentration in gastric juices from patients before and after the H. pylori eradication. *p \ 0.05; **p \ 0.01; NS not significant

    Journal: Digestive diseases and sciences

    Article Title: Pathophysiological investigation of the gastric surface mucous gel layer of patients with Helicobacter pylori infection by using immunoassays for trefoil factor family 2 and gastric gland mucous cell-type mucin in gastric juice.

    doi: 10.1007/s10620-011-1724-9

    Figure Lengend Snippet: Fig. 3 TFF2 concentration in gastric juices from healthy individuals and patients. a TFF2 concentration in gastric juices from patients with gastritis, gastric ulcer (GU), duodenal ulcer (DU) and healthy individuals (control). b TFF2 concentration in gastric juices from patients with H. pylori infection (HP (?)) and in H. pylori-negative patients (HP (-)). c TFF2 concentration in gastric juices from patients before and after the H. pylori eradication. *p \ 0.05; **p \ 0.01; NS not significant

    Article Snippet: The biotinylated anti-human TFF2 antibody was prepared by using the goat polyclonal anti-human TFF2 antibody (R&D systems) and the EZ-Link Sulfo-NHS-LC-Biotinylation kit (Pierce, Rockford, IL) according to the manufacturer’s instructions.

    Techniques: Concentration Assay, Control, Infection